This box of hair serums are new and never opened. The product is made in Italy and has niacinamide, zinc, and 3% redensyl for visibly thicker and fuller looking hair. The box contains 14 Ampoules and 2 reusable applicator tops. You apply 1/2 of an ampoule to freshly washed hair each time you wash it for the first month and then once a week for maintenance. I bought this to try and then started using Minoxidil instead, so you can give it a try for much less than I paid. Reviews for it look very good.